Separation of Vitamin B2 and B12 byImpregnate HPTLC Plates with Boric Acid
نویسندگان
چکیده
A high performance thin layer chromatography system (HPTLC) for the separation of vitamin B2 and B12 has been developed. The separation was successfully using a solvent system of methanol, water, ammonia 7.3.1 (V/V) as mobile phase on HPTLC plates impregnated with boric acid. The effect of other mobile phases on the separation of vitamins was also examined. The method is based on different behavior of investigated compounds in impregnated TLC plates with different amount of boric acid. The Rf values of vitamin B2 and B12 are considered on non impregnated and impregnated silica gel HPTLC plate with boric acid. The effect of boric acid in the mobile phase and on HPTLC plates on the RF values of the vitamins has also been studied. Keywords—High performance thin layer chromatography, HPTLC, Vitamin B2, Vitamin B12, Separation.
منابع مشابه
Unidimensional Thin Layer Chromatography of Salicylic Acid Derivatives on Boric Acid-Impregnated Plates in Drugs Studies
A unidimensional Thin Layer Chromatography system for the separation of salicylic acid and its derivatives (sulfosalicylic acid and Aspirin) has been developed. The separation was successfully using a solvent system of chloroform-methanol-water-ammonium hydroxide 120:75:6:2 (V/V) as mobile phase on TLC plates impregnated with boric acid. The method is based on different behavior of investigated...
متن کاملTHIN-LAYER CHROMATOGRAPHIC ANALYSIS OF HYDROPHILIC VITAMINS IN STANDARDS AND FROM Helisoma trivolvis SNAILS
Separations of the hydrophilic vitamins thiamine (B1), riboflavin (B2), niacin (B3), pyridoxine (B6), cobalamin (B12), ascorbic acid (C), and folic acid have been compared using 14 mobile phases and commercially available, precoated silica gel and chemically bonded silica gel thin-layer chromatography (TLC) and high-performance thin-layer chromatography (HPTLC) plates. The best separations of i...
متن کاملSerum levels of vitamin B12, folic acid, and homocysteine in patients with vitiligo
Background and Aim: Vitiligo is a hypopigmentation disorder of the skin that is associated with depression and an impaired quality of life. There has been conflicting reports on the association between the disease and the serum levels of homocysteine, vitamin B12, and folic acid.In this study,serum levels of homocysteine, vitamin B12, and folic acid were evaluated in patients with vitiligo.Meth...
متن کاملBasic principles of TLC
Products for thin layer chromatography Ready-to-use layers for TLC Summary of MN ready-to-use layers for TLC 263 – 265 TLC ready-to-use layers with standard silica 266 – 269 TLC ready-to-use layers with concentrating zone 269 HPTLC ready-to-use layers with nano silica 269 – 271 Nano plates with extremely thin silica layer 271 TLC and HPTLC plates with modified silica layers 272 – 277 HPTLC read...
متن کاملEffects of Vitamin B12 in Neonates and Young Infants
Vitamin B12 (cobalamin) is an essential coenzyme for nucleic acid synthesis. Animal protein is the major dietary source of vitamin B12. Deficiency of vitamin B12 leads to megaloblastic anemia, degeneration of the brain, spinal cord, peripheral nerves, and abnormalities of epithelial tissues. Two factors are necessary for the cure of megaloblastic anemia: one in food (extrinsic factor) and one i...
متن کامل